The idea behind the project is to develop technologies and tools for rapid diagnosis by implementing a Point-Of-Care (POC) platform based on LOC technology to isolate, concentrate and amplify viral RNA through an innovative and multidisciplinary approach involving microfluidics, nanomaterials and functionalization of polymers. The ultimate goal of RIVAL is to obtain a system that from a polymeric card incorporating a microfluidic circuit and reagents is able to implement different functionalities that correspond to specific separation, concentration and amplification steps, namely: the isolation of the SARS-COV2 virus and capture on the functionalized surface to obtain reduced volume and concentration; RNA capture (extraction and immobilization on a high-surface area covered with nanomaterials); RNA amplification (release and amplification with an isothermal method (“Reverse transcription loop-mediated isothermal amplification”, RT-LAMP)).
